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  Indian J Med Microbiol
 

Deletion of metacaspase enhances protein carbonylation in Saccharomyces cerevisiae. One-dimensional (a) and two-dimensional (b) immunoblot analyses of carbonylated proteins using 2,4-dinitrophenyl antibody. Proteins were identified by MALDI-TOF/TOF MS by excising protein spots manually from the second 2D gel run in parallel, stained with Coomassie Brilliant Blue-R250 as described in “Materials and Methods.” Differential migration patterns of carbonylated proteins in terms of IEF and molecular weight shift are mapped in grid graphs and compared (c). H2O2, hydrogen peroxide; WT, wild type.

Deletion of metacaspase enhances protein carbonylation in <i>Saccharomyces cerevisiae</i>. One-dimensional (a) and two-dimensional (b) immunoblot analyses of carbonylated proteins using 2,4-dinitrophenyl antibody. Proteins were identified by MALDI-TOF/TOF MS by excising protein spots manually from the second 2D gel run in parallel, stained with Coomassie Brilliant Blue-R250 as described in “Materials and Methods.” Differential migration patterns of carbonylated proteins in terms of IEF and molecular weight shift are mapped in grid graphs and compared (c). H<sub>2</sub>O<sub>2</sub>, hydrogen peroxide; WT, wild type.