International Journal of Nutrition, Pharmacology, Neurological Diseases

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 3  |  Page : 112--119

The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7


Rohman Mohammad Saifur1, Sishartami Lintang Widya2, Widodo Nashi3, Rachmawati Ermin4, Lukitasari Mifetika5 
1 Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia; Brawijaya Cardiovascular Research Centre, Universitas Brawijaya, Malang, East Java, Indonesia
2 Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya; Department of Public Health, Faculty of Sport Science, Universitas Negeri Malang, Malang, East Java, Indonesia
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, East Java, Indonesia
4 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, Malang, East Java, Indonesia
5 Brawijaya Cardiovascular Research Centre, Universitas Brawijaya, Malang, East Java; School of Nursing, Faculty of Health Sciences, Universitas Brawijaya, Malang, East Java, Indonesia

Correspondence Address:
Sishartami Lintang Widya
Faculty of Sport Science, Semarang Street Number 5, Universitas Negeri Malang, Malang City, East Java 65145, Indonesia
Indonesia

Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 μg/mL + green tea 80 μg/mL; coffee 160 μg/mL + green tea 160 μg/mL; coffee 320 μg/mL + green tea 320 μg/mL), respectively. Then, cells stimulated with 8 μg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells.


How to cite this article:
Mohammad Saifur R, Lintang Widya S, Nashi W, Ermin R, Mifetika L. The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7.Int J Nutr Pharmacol Neurol Dis 2022;12:112-119


How to cite this URL:
Mohammad Saifur R, Lintang Widya S, Nashi W, Ermin R, Mifetika L. The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7. Int J Nutr Pharmacol Neurol Dis [serial online] 2022 [cited 2022 Dec 8 ];12:112-119
Available from: https://www.ijnpnd.com/article.asp?issn=2231-0738;year=2022;volume=12;issue=3;spage=112;epage=119;aulast=Mohammad;type=0