International Journal of Nutrition, Pharmacology, Neurological Diseases

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 2  |  Page : 39--45

Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae


Mohammed Abdul Sattar Khan1, Meghan Morgan2, Musthafa Mohamed Essa3, Mohammed Akbar4, Gilles J. Guillemin5, Byoung-Joon Song4 
1 Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
2 Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
3 Department of Food Science and Nutrition, College of Agriculture and Marine Sciences, Sultan Qaboos University, Seeb; Ageing and Dementia Research Group, Sultan Qaboos University, Seeb, Oman
4 Section of Molecular Pharmacology and Toxicology, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism (NIAAA), Bethesda, MD, USA
5 Neuroinflammation Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia

Correspondence Address:

Hydrogen peroxide (H2O2) is an oxidant which could induce posttranslational modifications of proteins (PTMPs) in cells. It is still unknown that carbonylated proteins (CPs) were accumulated in caspase-suppressed leukemia cells or caspase-deleted Saccharomyces cerevisiae (yeast). Hence, we aimed to identify CPs and elucidate the role of metacaspase in regulating PTMPs and identify/compare the differentially expressed PTMPs in Δyca1 mutant compared to wild type with/without H2O2 exposure by proteomics approach. We found that deletion of the metacaspase gene (MCG) in yeast resulted in accumulation of high amounts of PTMPs associated with protein synthesis and carbohydrate metabolism compared to H2O2, which suggests that MCG is involved in the regulation of PTMPs and it could protect yeast from oxidative stress.


How to cite this article:
Khan MS, Morgan M, Essa M, Akbar M, Guillemin GJ, Song BJ. Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae.Int J Nutr Pharmacol Neurol Dis 2022;12:39-45


How to cite this URL:
Khan MS, Morgan M, Essa M, Akbar M, Guillemin GJ, Song BJ. Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae. Int J Nutr Pharmacol Neurol Dis [serial online] 2022 [cited 2022 Sep 25 ];12:39-45
Available from: https://www.ijnpnd.com/article.asp?issn=2231-0738;year=2022;volume=12;issue=2;spage=39;epage=45;aulast=Khan;type=0