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ORIGINAL ARTICLE
Year : 2022  |  Volume : 12  |  Issue : 3  |  Page : 112-119

The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7


1 Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia; Brawijaya Cardiovascular Research Centre, Universitas Brawijaya, Malang, East Java, Indonesia
2 Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya; Department of Public Health, Faculty of Sport Science, Universitas Negeri Malang, Malang, East Java, Indonesia
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, East Java, Indonesia
4 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, Malang, East Java, Indonesia
5 Brawijaya Cardiovascular Research Centre, Universitas Brawijaya, Malang, East Java; School of Nursing, Faculty of Health Sciences, Universitas Brawijaya, Malang, East Java, Indonesia

Correspondence Address:
Sishartami Lintang Widya
Faculty of Sport Science, Semarang Street Number 5, Universitas Negeri Malang, Malang City, East Java 65145, Indonesia
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijnpnd.ijnpnd_7_22

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Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 μg/mL + green tea 80 μg/mL; coffee 160 μg/mL + green tea 160 μg/mL; coffee 320 μg/mL + green tea 320 μg/mL), respectively. Then, cells stimulated with 8 μg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells.


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